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scaffold 其实是基因组组装时的概念。涉及到的相关概念如下： reads：就是我们测序产生的短读序列，通常一代和三代的reads读长在几千到几万bp之间，二代的相对较短，平均是几十到几百bp。 contig：中文叫做重叠群，就是不同reads之间的overlap交叠区，拼接成的序列就是contig scaffold: 是比contig还要长的. scaffoldというのは足場という他に骨格という意味合いがありますので、 化学用語に則ると 多孔質構造 構造に適した新しいグループ みたいな感じに直訳できます。 多孔質骨格は用語としておかしいので構造でいいと思われます。 なるほど 0 そうだね 0. This guide provides information on managing the risks with scaffolds and scaffolding at the workplace
This guide is supported by specific guidance material for It is part of a series of guidance material and should be read and used together with the general guide for scaffolds and scaffolding work which includes information on risk management as well as advice on planning, erecting, altering, dismantling and working with scaffolds and the following material: Suspended (swing stage) scaffolds scaffold inspection and maintenance tower and mobile scaffolds, and scaffolding work near overhead electric lines.
This guide provides information on scaffold inspection and maintenance including information on certification that a scaffold is safe to use.
scaffold直接翻译过来是脚手架。 这个概念和“测末端”有关。 简单地说，你测序的时候筛选到了20kb长的片段，但是一代和二代都测不通，只能测两端很短的序列。 这时候你有了这个20kb的片段两端的序列，以及这两段序列之间的距离（20k）。 tracrRNA（在CRISPR-Cas9编辑技术中被优化并命名为gRNA scaffold），它负责与Cas9结合，与重复序列具有同源性。 crRNA为引导序列，约20个碱基，具有特异性。 其中，crRNA和tracrRNA通过局部碱基配对组合并与Cas9结合后，引导Cas9识别切割目标DNA序列 (图1)。 This general guide provides information on how to manage risks associated with scaffolds and scaffolding work at a workplace It is supported by guidance material for specific types of scaffolds and scaffolding, suspended (swing stage) scaffolds, scaffold inspection and maintenance, and advice for small businesses and workers on managing the risks associated with tower and mobile scaffolds and.
Construction work commonly includes scaffolds They provide a safe, stable work platform when working at heights Scaffolding is the individual components that, when assembled, form a scaffold For example, tubes, couplers and frames
Scaffolding work means erecting, altering or dismantling a temporary structure that supports a platform.
Scaffolding work means erecting, altering or dismantling a temporary structure that supports a platform The guide to scaffolds and scaffolding has information on types of scaffolds and their uses
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