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La tinción de gram se utiliza para diferenciar las bacterias intactas y morfológicamente similares en dos grupos, según el color de la célula después de la tinción Un elemento clave en muchos métodos de tinción es el uso de un contracolorante, que se aplica después del colorante principal para resaltar el contraste y mejorar la visibilidad de las estructuras celulares. Además, se hacen evidentes la forma, el tamaño y los detalles estructurales de la célula.
Emplean dos colorantes, al primero se le llama colorante primario, al segundo contracolorante o colorante de contraste Es una de las tinciones más fundamentales en microbiología. Generalmente existe un paso de decoloración e ntre ambos.
La tinción de gram es una técnica de laboratorio que permite diferenciar las especies bacterianas en dos grupos según la estructura de sus paredes celulares
Esta prueba es fundamental para identificar las bacterias y determinar el tratamiento adecuado, especialmente cuando se necesitan antibióticos. La tinción utiliza un colorante rojo que tiñe las bacterias ácido alcohol resistentes de rojo, mientras que un contracolorante azul tiñe otras bacterias de azul.
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